Thyroid Peroxidase Antibody (Anti-TPO)
Test ID: A869
Thyroid peroxidase is a protein produced from cells in the thyroid gland. Thyroid peroxidase antibodies (anti-TPO) occur when the body’s own immune system attacks the thyroid and targets thyroid peroxidase. This occurs in autoimmune disorders targeting the thyroid gland, such as Hashimoto’s thyroiditis (causing hypothyroidism) and Graves’ disease (causing hyperthyroidism).
How to order a test
- Thyroid Peroxidase Autoantibodies
- TPO Antibodies
- Antimicrosomal Antibody
- Antithyroid Microsomal Antibody
What is Included?
Measurement of the IgG class of thyroid peroxidase autoantibodies (anti-TPO) in a blood sample by chemiluminescent microparticle immunoassay.
1 – 3 business days
The turnaround time is not guaranteed. The average turnaround time is 1 – 3 business days from the date that the sample arrives at the laboratory. Shipping time for the sample is not included. Additional time is required if the case requires confirmatory or reflex testing, or if the sample is insufficient, or if a recollection is required.
Additional Information and Resources
Preparation Before Specimen Collection
50 μL in a microtainer
Microtainer (regular blood tube)
This test requires a blood sample from a finger prick. All supplies for sample collection are provided in the kit.
- First wash and dry hands. Warm hands aid in blood collection.
- Clean the finger prick site with the alcohol swab and allow to air dry.
- Use the provided lancet to puncture the skin in one quick, continuous and deliberate stroke.
- Wipe away the first drop of blood.
- Massage hand and finger to increase blood flow to the puncture site. Angle arm and hand downwards to facilitate blood collection on the fingertip.
- Drip blood into the microtainer tube.
- Dispose of all sharps safely and return sample to the laboratory in the provided prepaid return shipping envelope.
NOTES: Avoid squeezing or ‘milking’ the finger excessively. If more blood is required and blood flow stops, perform a second skin puncture on another finger. Do not touch the fingertip.
Maintain specimen at temperatures between 2°C and 30°C during storage and transport.
Blood samples can be refrigerated or kept at room temperature for up to 7 days.
Causes for Rejection
- Incorrect or incomplete patient identification
- Incorrect specimen collection
- Inappropriate storage and transport conditions
- Incorrect specimen volume
To measure thyroid peroxidase autoantibodies (anti-TPO) levels in a blood sample for the evaluation of thyroid function and diagnosis of thyroid disease.
- This report is not intended for use in medico-legal applications.
- These results should be interpreted in conjunction with other laboratory and clinical information.
- Correct specimen collection and handling is required for optimal assay performance.
- Interferences from medication or endogenous substances may affect results.
- The presence of anti-TPO antibodies does not confirm autoimmune thyroid disease or abnormal thyroid function. Anti-TPO antibodies are frequently found in other autoimmune diseases, including Rheumatoid Arthritis, Addison’s Disease and Type I Diabetes, and may occur in up to 20% of asymptomatic individuals.
Chemiluminescent microparticle immunoassay (Alinity i Anti-TPO assay)
Normal serum levels are < 5.61 IU/mL
This reference range was obtained from the Alinity i Anti-TPO package insert.